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The Binding Constant for Complexation of Bilirubin to Bovine Serum Albumin. An Experiment for the Biophysical Chemistry Laboratory

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Kathryn R. Williams , Bhavin Adhyaru and Russell E. Pierce

Department of Chemistry, University of Florida, Gainesville, FL 32611-7200

Stephen G. Schulman

Department of Medicinal Chemistry, University of Florida, Gainesville, FL 32610-0485

J. Chem. Educ., 2002, 79 (1), p 115

DOI: 10.1021/ed079p115

Publication Date (Web): January 1, 2002

Abstract

Students use fluorescence spectroscopy to measure the binding constant for bilirubin-bovine serum albumin complexation by the Method of Continuous Variations. The experiment applies fundamental concepts of physical chemistry to a commercially available system of biological interest. The experiment is easily completed in a single laboratory period, and students obtain binding constants on the order of 10 ⁷, in good agreement with literature values.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Laboratory Instruction

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Biophysical Chemistry

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