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Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories
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Abstract
Students measure the diffusion coefficient of dopamine (3-hydroxytyramine) by capillary electrophoresis using three evaluation methods: single-point, graphical, and stopped migration. The experiment combines instruction of modern instrumentation with the determination of a biologically significant physical constant.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Biophysical ChemistryCiting Articles
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This article has been cited by 5 ACS Journal articles (5 most recent appear below).
Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
Lisa A. HollandJournal of Chemical Education2011 88 (3), 254-256Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
Lisa A. HollandJournal of Chemical Education2011 88 (3), 254-256Capillary electrophoresis is a versatile technique well suited to teach concepts that are fundamental to a chemistry degree program. The applicability of capillary electrophoresis to metabolites, DNA, proteins, pharmaceutical compounds, environmental ...
An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection
Jonathan E. Thompson and Khriesto Shurrush , Greg AndersonJournal of Chemical Education2006 83 (11), 1677An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection
Jonathan E. Thompson and Khriesto Shurrush , Greg AndersonJournal of Chemical Education2006 83 (11), 1677We describe an inexpensive device for performing capillary electrophoresis (CE) separations with fluorescence detection. As a demonstration of the device's utility we have determined the mass of riboflavin in a commercially available dietary supplement. ...
Weak Acid pKa Determination Using Capillary Zone Electrophoresis
Mike SolowJournal of Chemical Education2006 83 (8), 1194Weak Acid pKa Determination Using Capillary Zone Electrophoresis
Mike SolowJournal of Chemical Education2006 83 (8), 1194This article describes a general chemistry experiment designed to measure the pKa of benzoic acid using capillary zone electrophoresis. Despite the fact that modern biology relies so heavily on this analytical technique, students get very little ...
The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting
Peihong Liang , Bhavin Adhyaru , Wright L. Pearson and Kathryn R. WilliamsJournal of Chemical Education2006 83 (2), 294The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting
Peihong Liang , Bhavin Adhyaru , Wright L. Pearson and Kathryn R. WilliamsJournal of Chemical Education2006 83 (2), 294Students determine the binding constant of estradiol to bovine serum albumin using 3H-labeled estradiol. The estradiol is equilibrated with excess BSA, and subsequently a small fraction (ca. 1%) of the unbound hormone is extracted using solid-phase ...
Removal of Zinc from Carbonic Anhydrase. A Kinetics Experiment for Upper-Level Chemistry Laboratories
Kathryn R. Williams and Bhavin AdhyaruJournal of Chemical Education2004 81 (7), 1045Removal of Zinc from Carbonic Anhydrase. A Kinetics Experiment for Upper-Level Chemistry Laboratories
Kathryn R. Williams and Bhavin AdhyaruJournal of Chemical Education2004 81 (7), 1045The Zn(II) ion in the active site of carbonic anhydrase can be removed by complexation with 2,6-pyridinedicarboxylate (dipicolinate). The kinetics of Zn(II) removal can be followed by two methods. In the standard experiment (suitable for students in ...
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- Received: August 03, 2009
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