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Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories
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Abstract
Students measure the diffusion coefficient of dopamine (3-hydroxytyramine) by capillary electrophoresis using three evaluation methods: single-point, graphical, and stopped migration. The experiment combines instruction of modern instrumentation with the determination of a biologically significant physical constant.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Biophysical ChemistryCiting Articles
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This article has been cited by 6 ACS Journal articles (5 most recent appear below).
Determination of Molecular Self-Diffusion Coefficients Using Pulsed-Field-Gradient NMR: An Experiment for Undergraduate Physical Chemistry Laboratory
Jennifer Harmon, Cierra Coffman, Spring Villarrial, Steven Chabolla, Kurt A. Heisel, and Viswanathan V. KrishnanJournal of Chemical Education2012 89 (6), 780-783Determination of Molecular Self-Diffusion Coefficients Using Pulsed-Field-Gradient NMR: An Experiment for Undergraduate Physical Chemistry Laboratory
Jennifer Harmon, Cierra Coffman, Spring Villarrial, Steven Chabolla, Kurt A. Heisel, and Viswanathan V. KrishnanJournal of Chemical Education2012 89 (6), 780-783NMR spectroscopy has become one of the primary tools that chemists utilize to characterize a range of chemical species in the solution phase, from small organic molecules to medium-sized proteins. A discussion of NMR spectroscopy is an essential component ...
Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
Lisa A. HollandJournal of Chemical Education2011 88 (3), 254-256Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
Lisa A. HollandJournal of Chemical Education2011 88 (3), 254-256Capillary electrophoresis is a versatile technique well suited to teach concepts that are fundamental to a chemistry degree program. The applicability of capillary electrophoresis to metabolites, DNA, proteins, pharmaceutical compounds, environmental ...
An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection
Jonathan E. Thompson and Khriesto Shurrush , Greg AndersonJournal of Chemical Education2006 83 (11), 1677An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection
Jonathan E. Thompson and Khriesto Shurrush , Greg AndersonJournal of Chemical Education2006 83 (11), 1677We describe an inexpensive device for performing capillary electrophoresis (CE) separations with fluorescence detection. As a demonstration of the device's utility we have determined the mass of riboflavin in a commercially available dietary supplement. ...
Weak Acid pKa Determination Using Capillary Zone Electrophoresis
Mike SolowJournal of Chemical Education2006 83 (8), 1194Weak Acid pKa Determination Using Capillary Zone Electrophoresis
Mike SolowJournal of Chemical Education2006 83 (8), 1194This article describes a general chemistry experiment designed to measure the pKa of benzoic acid using capillary zone electrophoresis. Despite the fact that modern biology relies so heavily on this analytical technique, students get very little ...
The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting
Peihong Liang , Bhavin Adhyaru , Wright L. Pearson and Kathryn R. WilliamsJournal of Chemical Education2006 83 (2), 294The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting
Peihong Liang , Bhavin Adhyaru , Wright L. Pearson and Kathryn R. WilliamsJournal of Chemical Education2006 83 (2), 294Students determine the binding constant of estradiol to bovine serum albumin using 3H-labeled estradiol. The estradiol is equilibrated with excess BSA, and subsequently a small fraction (ca. 1%) of the unbound hormone is extracted using solid-phase ...
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- Received: August 03, 2009
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