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Factors affecting reaction kinetics of glucose oxidase

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Kristin A. Johnson

Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706

Beth A. Kroa and Tony Yourey

Department of Chemisty, Kutztown University, Kutztown, PA 19530

J. Chem. Educ., 2002, 79 (1), p 74

DOI: 10.1021/ed079p74

Publication Date (Web): January 1, 2002

Abstract

Basic principles of enzyme kinetics are demonstrated using the enzyme glucose oxidase. The glucose oxidase enzymatic reaction is coupled to horseradish peroxidase, which in turn catalyzes the oxidation of a dye to a bright blue-green color. The appearance of the blue-green dye is used to monitor the course of the reaction and is quite visible in a classroom setting. A series of reactions are arranged that vary the enzyme concentration, substrate concentration, temperature, and the substrate used in the reaction. By monitoring the rate of the color change in each beaker, the reaction kinetics of glucose oxidase in each series is observed.

Keywords (Audience):

High School / Introductory Chemistry

Keywords (Domain):

Biochemistry

Keywords (Feature):

Tested Demonstrations

Keywords (Subject):

Enzymes

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