Application of Datalogger in Biosensing: A Glucose Biosensor

Martin M. F. Choi and Pui Shan Wong
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, SAR, PRC
J. Chem. Educ., 2002, 79 (8), p 982
DOI: 10.1021/ed079p982
Publication Date (Web): August 1, 2002

Abstract

This article describes the use of a datalogger in conjunction with an oxygen sensor to fabricate a glucose biosensor. Glucose oxidase is first covalently immobilized on an eggshell membrane. The membrane is then used to cover the surface of an oxygen electrode. The detection scheme is based on the depletion of the dissolved oxygen upon exposure to glucose solution. The decrease in oxygen level is monitored and related to the glucose concentration. This biosensor has been successfully applied to determine the glucose content in beverage samples. These experiments provide suitable hands-on experience for undergraduate and postgraduate students to help them understand biosensors.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Computer-Based Learning

Keywords (Subject):

Carbohydrates

Citing Articles

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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

  • Cover Image

    Crystallization of β-d-Glucose and Analysis with a Simple Glucose Biosensor

    José I. Reyes-de-Corcuera , Michael A. Teruel and Daniel M. Jenkins
    Journal of Chemical Education2009 86 (8), 959
    • Crystallization of β-d-Glucose and Analysis with a Simple Glucose Biosensor

      José I. Reyes-de-Corcuera , Michael A. Teruel and Daniel M. Jenkins
      Journal of Chemical Education2009 86 (8), 959

      A simple method to crystallize β-D-glucose using reagents and equipment common to any chemistry laboratory is described. The crystals are analyzed with a glucose electrode prepared by entrapping glucose oxidase in a poly-ortho-phenylenediamine film on a ...

  • Cover Image

    Homemade Bienzymatic-Amperometric Biosensor for Beverages Analysis

    M. C. Blanco-López , M. J. Lobo-Castañón and A. J. Miranda-Ordieres
    Journal of Chemical Education2007 84 (4), 677
    • Homemade Bienzymatic-Amperometric Biosensor for Beverages Analysis

      M. C. Blanco-López , M. J. Lobo-Castañón and A. J. Miranda-Ordieres
      Journal of Chemical Education2007 84 (4), 677

      In this experiment students construct an amperometric glucose biosensor and use it to determine the glucose concentration in soft drinks. This experiment is appropriate for an advanced instrumental course; however, it could be adapted to elementary ...

  • Cover Image

    Application of a Datalogger in Biosensing: A Reagentless Hydrogen Peroxide Biosensor

    Lihua Ma and Martin M. F. Choi
    Journal of Chemical Education2004 81 (6), 862
    • Application of a Datalogger in Biosensing: A Reagentless Hydrogen Peroxide Biosensor

      Lihua Ma and Martin M. F. Choi
      Journal of Chemical Education2004 81 (6), 862

      The article describes the use of a datalogger in conjunction with an oxygen sensor to fabricate a hydrogen peroxide biosensor. An eggshell membrane is peeled from a fresh egg. The membrane is used to cover the surface of an oxygen electrode. The detection ...

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History

  • Received: August 03, 2009

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