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Application of Datalogger in Biosensing: A Glucose Biosensor
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Abstract
This article describes the use of a datalogger in conjunction with an oxygen sensor to fabricate a glucose biosensor. Glucose oxidase is first covalently immobilized on an eggshell membrane. The membrane is then used to cover the surface of an oxygen electrode. The detection scheme is based on the depletion of the dissolved oxygen upon exposure to glucose solution. The decrease in oxygen level is monitored and related to the glucose concentration. This biosensor has been successfully applied to determine the glucose content in beverage samples. These experiments provide suitable hands-on experience for undergraduate and postgraduate students to help them understand biosensors.
Keywords (Audience):
Second-Year UndergraduateKeywords (Domain):
BiochemistryKeywords (Pedagogy):
Computer-Based LearningKeywords (Subject):
CarbohydratesCiting Articles
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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

Crystallization of β-d-Glucose and Analysis with a Simple Glucose Biosensor
José I. Reyes-de-Corcuera , Michael A. Teruel and Daniel M. JenkinsJournal of Chemical Education2009 86 (8), 959Crystallization of β-d-Glucose and Analysis with a Simple Glucose Biosensor
José I. Reyes-de-Corcuera , Michael A. Teruel and Daniel M. JenkinsJournal of Chemical Education2009 86 (8), 959A simple method to crystallize β-D-glucose using reagents and equipment common to any chemistry laboratory is described. The crystals are analyzed with a glucose electrode prepared by entrapping glucose oxidase in a poly-ortho-phenylenediamine film on a ...

Homemade Bienzymatic-Amperometric Biosensor for Beverages Analysis
M. C. Blanco-López , M. J. Lobo-Castañón and A. J. Miranda-OrdieresJournal of Chemical Education2007 84 (4), 677Homemade Bienzymatic-Amperometric Biosensor for Beverages Analysis
M. C. Blanco-López , M. J. Lobo-Castañón and A. J. Miranda-OrdieresJournal of Chemical Education2007 84 (4), 677In this experiment students construct an amperometric glucose biosensor and use it to determine the glucose concentration in soft drinks. This experiment is appropriate for an advanced instrumental course; however, it could be adapted to elementary ...

Application of a Datalogger in Biosensing: A Reagentless Hydrogen Peroxide Biosensor
Lihua Ma and Martin M. F. ChoiJournal of Chemical Education2004 81 (6), 862Application of a Datalogger in Biosensing: A Reagentless Hydrogen Peroxide Biosensor
Lihua Ma and Martin M. F. ChoiJournal of Chemical Education2004 81 (6), 862The article describes the use of a datalogger in conjunction with an oxygen sensor to fabricate a hydrogen peroxide biosensor. An eggshell membrane is peeled from a fresh egg. The membrane is used to cover the surface of an oxygen electrode. The detection ...
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History
- Received: August 03, 2009
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