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Simulating Chromatographic Separations in the Classroom
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Abstract
A classroom activity has been developed that allows students to perform chromatographic separations in the classroom through active participation. Using this classroom simulation, students develop a set of rules used to investigate the effect of flow rate, column dimension, particle size, and mobile/stationary phase affinity on chromatographic separations. While performing the simulation, the concepts of longitudinal and eddy diffusion are dramatically illustrated. Students are not only active participants throughout the entire separation; they also directly observe an evolving separation. The simulation allows students to set initial parameters, watch, and directly participate in isocratic and gradient separations occurring in real time.
Keywords (Audience):
First-Year Undergraduate / GeneralKeywords (Domain):
Analytical ChemistryKeywords (Subject):
ChromatographyCiting Articles
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This article has been cited by 3 ACS Journal articles (3 most recent appear below).
Board-Game Gel Filtration and Affinity Chromatography
Eric A. Hunt and Sapna K. DeoJournal of Chemical Education2009 86 (1), 19Board-Game Gel Filtration and Affinity Chromatography
Eric A. Hunt and Sapna K. DeoJournal of Chemical Education2009 86 (1), 19The gel filtration and affinity chromatography board game activity described here is a simulation of common chromatographic techniques. The game is suitable for use in a classroom or laboratory setting. This activity utilizes a paper grid, colored paper ...
Separation Anxiety: An In-Class Game Designed To Help Students Discover Chromatography
Michael J. SamideJournal of Chemical Education2008 85 (11), 1512Separation Anxiety: An In-Class Game Designed To Help Students Discover Chromatography
Michael J. SamideJournal of Chemical Education2008 85 (11), 1512An in-class activity has been developed to assist students in discovering chromatographic separations. Designed on the basis of plate theory, the game has students use instructor-provided partition coefficients to establish equilibrium for two different ...
Size Exclusion Chromatography: An Experiment for High School and Community College Chemistry and Biotechnology Laboratory Programs
Kathryn K. Davis , Linda S. BrunauerJournal of Chemical Education2008 85 (5), 683Size Exclusion Chromatography: An Experiment for High School and Community College Chemistry and Biotechnology Laboratory Programs
Kathryn K. Davis , Linda S. BrunauerJournal of Chemical Education2008 85 (5), 683A simple multiday laboratory exercise suitable for use in a high school or community college chemistry course or a biotechnology advanced placement biology course is described. In this experiment students gain experience in the use of column ...
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- Received: August 03, 2009
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