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A Fluorometric Approach to Studying the Effects of Ionic Strength on Reaction Rates: An Undergraduate Steady-State Fluorescence Laboratory Experiment

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Stephen W. Bigger and Peter J. Watkins

School of Molecular Sciences, Victoria University of Technology, Werribee Campus, Melbourne, 8001, Australia

Bruce Verity

School of Chemical Technology, University of South Australia, Mawson Lakes, 5095, Australia

J. Chem. Educ., 2003, 80 (10), p 1191

DOI: 10.1021/ed080p1191

Publication Date (Web): October 1, 2003

Abstract

A simple, steady-state fluorescence experiment is described in which the dynamic quenching of the quinine dication fluorescence by Cl^- and Br^- is studied. Solutions of sufficiently low ionic strength enable ionic activities to be taken into account by means of the Debye–Hückel equation. The experiment demonstrates how the rate of a reaction depends on the ionic strength of the medium. The experiment represents a unique example of the application of the Debye–Hückel equation without the use of simplifying assumptions regarding the calculation of ionic activity coefficients. The experiment also shows how the observed decrease in the Stern–Volmer rate constant with increasing ionic strength can be quantitatively modeled to determine the relative efficiencies of Cl^- and Br^- fluorescent quenching of quinine.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Laboratory Instruction

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Fluorescence Spectroscopy

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