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Using NMR to Determine Protein Structure in Solution

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Silvia Cavagnero

Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706

J. Chem. Educ., 2003, 80 (2), p 125

DOI: 10.1021/ed080p125

Publication Date (Web): February 1, 2003

Abstract

Nuclear magnetic resonance (NMR) is a marvelous spectroscopic technique that chemists, physicists, and biochemists routinely employ for their research around the world. This year half of the Nobel Prize for chemistry went to Kurt Wüthrich, who was recognized for the development of NMR-based techniques that lead to the structure determination of biomolecules in solution. In addition to implementing novel pulse sequences and software packages, Wüthrich also applied his methods to several biological systems of key importance to human health. These include the prion protein, which is heavily involved in the spongiform encephalopathy (best known as 'mad cow disease'), which recently caused numerous human deaths, particularly in the UK, due to ingestion of contaminated meat. Transverse relaxation optimized spectroscopy (TROSY) is the most intriguing new NMR method recently developed by Wüthrich and coworkers. This and other closely related pulse sequences promise to play a pivotal role in the extension of NMR to the conformational analysis of very large (up to the megadalton range) macromolecules and macromolecular complexes. More exciting new developments are expected in the near future.

Keywords (Audience):

General Public

Keywords (Domain):

Analytical Chemistry

Keywords (Feature):

Report: Nobel Prize in Chemistry, 2002

Keywords (Subject):

Molecular Properties / Structure

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

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- Confirming the 3D Solution Structure of a Short Double-Stranded DNA Sequence Using NMR Spectroscopy
  Rasha A. Ruhayel and Susan J. Berners-Price
  Journal of Chemical Education2010 87 (7), 732-734
  2D 1H NOESY NMR spectroscopy is routinely used to give information on the closeness of hydrogen atoms through space. This work is based on a 2D 1H NOESY NMR spectrum of a 12 base-pair DNA duplex. This 6-h laboratory workshop aims to provide advanced-level ...
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