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Simple Experiments To Demonstrate Proton Flux in Pseudomonas after Alkaline or Acidic Stress

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Gabriela Previtali , Walter Giordano and Carlos E. Domenech

Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Córdoba, Argentina

J. Chem. Educ., 2003, 80 (12), p 1468

DOI: 10.1021/ed080p1468

Publication Date (Web): December 1, 2003

Section:

History, Education, and Documentation

Abstract

This laboratory introduces chemistry students to the ability of microorganisms to adapt to acidic or alkaline environmental conditions. A laboratory experiment to ascertain the bacterial response to the stress produced by suspension in different pH solutions has been developed. The experiment may be performed in several versions depending on the availability of lab equipment and the chemistry level of the students. This laboratory experiment has the pedagogical advantage of giving chemistry students experience with the application of various pH levels to a biological system and enables the students to expand their understanding of pH to mean more than a strictly chemical concept.