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Fluorescence, Absorption, and Excitation Spectra of Polycyclic Aromatic Hydrocarbons as a Tool for Quantitative Analysis
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Abstract
An experiment suitable for college junior or senior students in the analytical instrumental analysis laboratory that demonstrates the analysis of PAHs (benzo[a]anthracene, benzo[k]fluoranthene, benzo[a]pyrene, chrysene, and phenanthrene) using absorption and fluorescence spectroscopy is described. This experiment is carried out during one seven-hour instrumental analysis laboratory. It could also be used in a physical chemistry laboratory to demonstrate fundamental spectroscopic and photochemical principles. A Beer–Lambert plot for an absorption peak of each PAH was obtained and used to determine the molar absorptivities. The effect of heavy atoms as quenchers of fluorescence was studied by using 1-bromoheptane and 1,7-dibromoheptane, and Stern–Volmer plots were prepared to determine the ratios of the quenching rate constants to the fluorescence rate constant, kQ/kf. The experiment is also useful as an experiment preceding the determination of PAHs by HPLC with absorption and fluorescence detection as described earlier (J. Chem. Educ. 1998, 75, 1599).
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Fluorescence SpectroscopyCiting Articles
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This article has been cited by 4 ACS Journal articles (4 most recent appear below).
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Grazyna Wilczek-Vera and Eric Dunbar SalinJournal of Chemical Education2011 88 (2), 216-219An experiment on fluorescence spectroscopy suitable for an advanced analytical laboratory is presented. Its conceptual development used a combination of the expository and discovery styles. The “learn-as-you-go” and direct “hands-on” methodology applied ...
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Barbara Branchi, Vincenzo Balzani, Paola Ceroni, Mireia Campañá Kuchenbrandt, Frank-Gerrit Klärner, Dieter Bläser and Roland BoeseThe Journal of Organic Chemistry2008 73 (15), 5839-5851We have synthesized molecular clips 1 comprising (i) two benzo[k]fluoranthene sidewalls and (ii) a dimethylene-connected benzene bridge that carries two acetoxy (1a), hydroxy (1b), or methoxy (1c) substituents in the para position. Their NMR spectra, ...
Steady-State Fluorescence Anisotropy To Investigate Flavonoids Binding to Proteins
Christine M. Ingersoll and Christen M. StrolloJournal of Chemical Education2007 84 (8), 1313Steady-State Fluorescence Anisotropy To Investigate Flavonoids Binding to Proteins
Christine M. Ingersoll and Christen M. StrolloJournal of Chemical Education2007 84 (8), 1313A laboratory experiment using steady-state fluorescence polarization or anisotropy to determine the binding constant for a flavonoid–protein interaction is described. Using the intrinsic fluorescence of quercetin (a model flavonoid), the fluorescence ...
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- Received: August 03, 2009
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