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Determination of Pb in Biological Samples by Graphite Furnace Atomic Absorption Spectrophotometry. An Exercise in Common Interferences and Fundamental Practices in Trace Element Determination

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Thomas M. Spudich and Jennifer K. Herrmann

School of Science, Penn State Erie, The Behrend College, Erie, PA 16563

Ronald Fietkau

Department of Chemistry and Physics, Georgia College and State University, Milledgeville, GA 31061

Grant A. Edwards and David L. McCurdy

Division of Science, Truman State University, Kirksville, MO 63501

J. Chem. Educ., 2004, 81 (2), p 262

DOI: 10.1021/ed081p262

Publication Date (Web): February 1, 2004

Section:

History, Education, and Documentation

Abstract

This laboratory experiment demonstrates the use of graphite furnace atomic absorption spectrophotometry (GFAAS) for the determination of trace levels of Pb in bovine muscle or liver samples. The major emphasis of the experiment is to explore common interferences, first by investigating the impact of molecular spectral absorption interferences on a real-world sample, then demonstrating the susceptibility of GFAAS to chemical–physical matrix interferences. The combination of D₂ arc background correction and calibration by standard additions is sufficient to reduce the magnitude of the interferences and obtain accurate Pb recoveries in these samples. This experiment also presents options for undergraduate chemistry majors to explore a number of other collateral issues related to the preparation of solid, biological samples for trace-level elemental determinations including sample decomposition, important figures of merit, and aspects of quality assurance in chemical measurements.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Analytical Chemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Atomic Spectroscopy

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

Quantitative Ultrasound-Assisted Extraction for Trace-Metal Determination: An Experiment for Analytical Chemistry
Isela Lavilla, Marta Costas, Francisco Pena-Pereira, Sandra Gil, and Carlos Bendicho
Journal of Chemical Education2011 88 (4), 480-483
- Quantitative Ultrasound-Assisted Extraction for Trace-Metal Determination: An Experiment for Analytical Chemistry
  Isela Lavilla, Marta Costas, Francisco Pena-Pereira, Sandra Gil, and Carlos Bendicho
  Journal of Chemical Education2011 88 (4), 480-483
  Ultrasound-assisted extraction (UAE) is introduced to upper-level analytical chemistry students as a simple strategy focused on sample preparation for trace-metal determination in biological tissues. Nickel extraction in seafood samples and quantification ...
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