Checkerboard Chromatography

This hands-on Activity allows students to simulate chromatographic simulations using a grid, colored pieces of paper, and a six-sided die. Students observe the separation as it is occurring and perform experiments on flow rate, column length, and mobile phase composition. This Activity is best performed after the class has discussed the topics of solubility and polarity. It can lead to discussions as to how separations are achieved in column chromatography. As the Activity progresses and students make observations, the instructor can introduce terminology including resolution, dead time, isocratic, gradient, and diffusion. The Activity is well suited for the high school or college level classroom where chromatographic concepts are being introduced.