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Fluorescence Microscopy of Single Molecules

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Jan Zimmermann , Arthur van Dorp and Alois Renn

Physical Chemistry Laboratory, Swiss Federal Institute of Technology, ETH Hoenggerberg, CH 8093 Zürich, Switzerland

J. Chem. Educ., 2004, 81 (4), p 553

DOI: 10.1021/ed081p553

Publication Date (Web): April 1, 2004

Section:

Radiation Chemistry, Photochemistry, and Photographic and Other Reprographic Processes

Abstract

A wide-field microscopy setup suitable for single-molecule detection at ambient temperature is described. The instrument, a lab-built inverted microscope that is operated in epi-fluorescence mode, allows the observation of fluorescing single molecules in real time. The instrument is used in an advanced laboratory course to investigate the influence of oxygen on molecular fluorescence at the single-molecule level. In this context students can be experimentally introduced into the world of single quantum systems.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Physical Chemistry

Keywords (Feature):

Topics in Chemical Instrumentation

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Dyes / Pigments

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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

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  Paul A. Flowers
  Journal of Chemical Education2011 88 (12), 1716-1719
  A simple and versatile approach to incorporating basic optical microscopy in the undergraduate instrumental analysis laboratory is described. Attaching a miniature CCD spectrometer to the video port of a standard compound microscope yields a visible ...
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Thermodynamic Exploration of Eosin−Lysozyme Binding
Andrew J. Huisman, Lydia R. Hartsell, Brent P. Krueger and Michael J. Pikaart
Journal of Chemical Education2010 87 (3), 299-302
- Thermodynamic Exploration of Eosin−Lysozyme Binding
  Andrew J. Huisman, Lydia R. Hartsell, Brent P. Krueger and Michael J. Pikaart
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  We developed a modular pair of experiments for use in the undergraduate physical chemistry and biochemistry laboratories. Both experiments examine the thermodynamics of the binding of a small molecule, eosin Y, to the protein lysozyme. The assay for ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
Observation of DNA Molecules Using Fluorescence Microscopy and Atomic Force Microscopy
Takashi Ito
Journal of Chemical Education2008 85 (5), 680
- Observation of DNA Molecules Using Fluorescence Microscopy and Atomic Force Microscopy
  Takashi Ito
  Journal of Chemical Education2008 85 (5), 680
  This article describes experiments for an undergraduate instrumental analysis laboratory that aim to observe individual double-stranded DNA (dsDNA) molecules using fluorescence microscopy and atomic force microscopy (AFM). dsDNA molecules are observed ...
  Abstract | Hi-Res PDF | PDF w/ Links