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Application of a Datalogger in Biosensing: A Reagentless Hydrogen Peroxide Biosensor

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Lihua Ma and Martin M. F. Choi

Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, SAR, China

J. Chem. Educ., 2004, 81 (6), p 862

DOI: 10.1021/ed081p862

Publication Date (Web): June 1, 2004

Section:

History, Education, and Documentation

Abstract

The article describes the use of a datalogger in conjunction with an oxygen sensor to fabricate a hydrogen peroxide biosensor. An eggshell membrane is peeled from a fresh egg. The membrane is used to cover the surface of an oxygen electrode. The detection scheme is based on the increase in dissolved oxygen content upon exposure to hydrogen peroxide solution. The increase in oxygen level is monitored and linearly related to hydrogen peroxide concentration. This biosensor has been successfully applied to determine the hydrogen peroxide concentration in some commercial samples. These experiments provide suitable simple hands-on experience for undergraduate and postgraduate students to help them understand the biosensor technology.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Analytical Chemistry

Keywords (Feature):

Computer Bulletin Board

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Laboratory Computing / Interfacing

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

A Quick and Accurate Oxygen-Based Pressure-Sensor Assay for Catalase Activity
Megan E. Lewis , Rebecca M. Levine , John T. York and W. Tandy Grubbs
Journal of Chemical Education2009 86 (10), 1227
- A Quick and Accurate Oxygen-Based Pressure-Sensor Assay for Catalase Activity
  Megan E. Lewis , Rebecca M. Levine , John T. York and W. Tandy Grubbs
  Journal of Chemical Education2009 86 (10), 1227
  A method is described for investigating the catalytic decomposition of hydrogen peroxide by using a computer-interfaced pressure sensor to follow the evolution of O2(g) inside a closed reaction vessel. The pressure sensor and accompanying glassware are ...
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