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Lipid Extraction and Cholesterol Quantification: A Simple Protocol
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Departmento de Ciências Tecnológicas e Desenvolvimento, Universidade dos Açores, 9502 Ponta Delgada, Portugal
J. Chem. Educ., 2005, 82 (1), p 103
DOI: 10.1021/ed082p103
Publication Date (Web): January 1, 2005
Abstract
A fast and simple protocol for lipid extraction from egg yolk and walnut is presented. Students learn to extract total lipids from both sources and to assess the quantity of cholesterol in lipid extracts by the Liebermann–Buchard method, in a semi-quantitative way. This experiment is easily done in a two-hour lab period, except for the evaporation of lipid extracts to dryness, which proceeds overnight and is weighed by the students the following day. Although enzymatic methods are currently used to measure cholesterol levels, the method described here has the advantage of being simple and quite inexpensive.
Keywords (Audience):
First-Year Undergraduate / GeneralKeywords (Domain):
BiochemistryKeywords (Pedagogy):
Humor / Puzzles / GamesKeywords (Subject):
Applications of ChemistryView: Hi-Res PDF | PDF w/ Links
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- Received: August 03, 2009
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