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Equilibrium Gel Filtration Chromatography for the Measurement of Protein-Ligand Binding in the Undergraduate Biochemistry Laboratory

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Douglas B. Craig

Department of Chemistry, University of Winnipeg, Winnipeg, MB R3B 2E9, Canada

J. Chem. Educ., 2005, 82 (1), p 96

DOI: 10.1021/ed082p96

Publication Date (Web): January 1, 2005

Section:

History, Education, and Documentation

Abstract

Equilibrium gel filtration liquid chromatography is a technique used for the quantitation of ligand binding. We have designed an experiment for the senior undergraduate laboratory using this technique for the measurement of the binding of ATP to cytochrome c. This experiment introduces the student to the use of liquid chromatography and absorbance spectrometry and permits a quantitative result within a three-hour period. The experiment complements classroom discussions regarding liquid chromatography, spectroscopy, dynamic equilibrium, and kinetic parameters.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Chromatography

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

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