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Fundamentals of Biomolecule Analysis by Electrospray Ionization Mass Spectrometry. An Instrumental Analysis Laboratory Experiment
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Abstract
This article describes an undergraduate instrumental analysis laboratory experiment dealing with fundamentals of electrospray ionization mass spectrometry (ESI–MS). Several important aspects of applications of ESI–MS for analysis of biomolecules are addressed. First, the formation of multiply charged ions and deconvolution of such ESI spectra to yield the molecular mass of the analyte is discussed. Second, the effects of isotopic contribution (mostly of 13C) are observed experimentally and verified by calculations. Last, the resolution of a mass spectrometer is probed as the ability to resolve individual peaks in isotopic clusters. Although the experiment is described for quadrupole ion trap mass spectrometers (two major manufacturers’ machines are tested here), it can be applied to virtually any ESI–MS instruments with minor modifications.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Instrumental MethodsCiting Articles
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This article has been cited by 7 ACS Journal articles (5 most recent appear below).
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Danny H. Kim, Curtis D. Eckhert, Kym F. FaullJournal of Chemical Education2011 88 (1), 106-110Mass spectrometry (MS) is a powerful analytical technique that is now widely used in the chemical, physical, engineering, and life sciences, with rapidly growing applications in many areas including clinical, forensic, pharmaceutical, and environmental ...
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Christopher J. Fenk, Nicole M. Hickman and Melissa A. Fincke, Douglas H. Motry, Barry LavineJournal of Chemical Education2010 87 (8), 838-841An undergraduate LC−MS experiment is described for the identification and quantitative determination of acetaminophen, acetylsalicylic acid, and caffeine in commercial analgesic tablets. This inquiry-based experimental procedure requires minimal sample ...
Identification of Fatty Acids, Phospholipids, and Their Oxidation Products Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry and Electrospray Ionization Mass Spectrometry
Christopher W. Harmon, Stephen A. Mang, John Greaves and Barbara J. Finlayson-PittsJournal of Chemical Education2010 87 (2), 186-189Identification of Fatty Acids, Phospholipids, and Their Oxidation Products Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry and Electrospray Ionization Mass Spectrometry
Christopher W. Harmon, Stephen A. Mang, John Greaves and Barbara J. Finlayson-PittsJournal of Chemical Education2010 87 (2), 186-189Electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) have found increasing application in the analysis of biological samples. Using these techniques to solve problems in analytical ...
Incorporating Biological Mass Spectrometry into Undergraduate Teaching Labs, Part 2: Peptide Identification via Molecular Mass Determination
Isaac J. Arnquist and Douglas J. BeussmanJournal of Chemical Education2009 86 (3), 382Incorporating Biological Mass Spectrometry into Undergraduate Teaching Labs, Part 2: Peptide Identification via Molecular Mass Determination
Isaac J. Arnquist and Douglas J. BeussmanJournal of Chemical Education2009 86 (3), 382Mass spectrometry has become a routine analytical tool in the undergraduate curriculum in the form of GC–MS. While relatively few undergraduate programs have incorporated biological mass spectrometry into their programs, the importance of these techniques,...
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- Received: August 03, 2009
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