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Label-Free Detection of DNA Hybridization by Cyclic Voltammetry. An Advanced Undergraduate Analytical Chemistry Laboratory Experiment
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Abstract
A label-free electrochemical detection method for DNA hybridization has been developed and tested in an advanced analytical chemistry course. A set of experiments was designed to explore several concepts relevant to electropolymerization of intrinsically conducting polymers, their use as an electrically controlled ion-exchanger of chloride ions, and their ability to bind oligonucleotides through a noncovalent interaction with magnesium ions used as a linker between the polymer and the DNA strand. The electrostatic barrier on the modified electrode created by the phosphate groups of the probe DNA attached to the magnesium ion is modified by DNA hybridization. Changes in the transfer rate of chloride ions of the "label-free DNA detector" are detected as variations in cyclic voltammograms. The basic approach for the detection of the DNA hybridization event relies on the subtraction of cyclic voltammograms recorded before and after DNA hybridization. Different aspects of the experiments can be emphasized, depending on the interests of students and the instructor.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Biophysical ChemistryCiting Articles
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This article has been cited by 3 ACS Journal articles (3 most recent appear below).
Label-Free Voltammetric Detection Using Individually Addressable Oligonucleotide Microelectrode Arrays
Roya Kalantari, Ryan Cantor, Hang Chen, George Yu, Jiri Janata, and Mira JosowiczAnalytical Chemistry2010 82 (21), 9028-9033Label-Free Voltammetric Detection Using Individually Addressable Oligonucleotide Microelectrode Arrays
Roya Kalantari, Ryan Cantor, Hang Chen, George Yu, Jiri Janata, and Mira JosowiczAnalytical Chemistry2010 82 (21), 9028-9033The utility and performance of label-free, oligonucleotide probes for reagentless detection of dilute target analytes was examined using a voltammetric transduction principle in an array format. Multistep, solid-state fabrication yielded preproduction ...
Gold Electrodes Modified with Self-Assembled Monolayers for Measuring L-Ascorbic Acid: An Undergraduate Analytical Chemistry Laboratory Experiment
Takashi Ito , D. M. Neluni T. Perera and Shinobu NagasakaJournal of Chemical Education2008 85 (8), 1112Gold Electrodes Modified with Self-Assembled Monolayers for Measuring L-Ascorbic Acid: An Undergraduate Analytical Chemistry Laboratory Experiment
Takashi Ito , D. M. Neluni T. Perera and Shinobu NagasakaJournal of Chemical Education2008 85 (8), 1112This article describes an undergraduate electrochemistry laboratory experiment in which the students measure the L-ascorbic acid content of a real sample. Gold electrodes modified with self-assembled monolayers (SAMs) of thioctic acid and cysteamine are ...
Label-Free DNA Detection of Hepatitis C Virus Based on Modified Conducting Polypyrrole Films at Microelectrodes and Atomic Force Microscopy Tip-Integrated Electrodes
Carla dos Santos Riccardi, Christine Kranz, Janusz Kowalik, Hideko Yamanaka, Boris Mizaikoff, and Mira JosowiczAnalytical Chemistry2008 80 (1), 237-245Label-Free DNA Detection of Hepatitis C Virus Based on Modified Conducting Polypyrrole Films at Microelectrodes and Atomic Force Microscopy Tip-Integrated Electrodes
Carla dos Santos Riccardi, Christine Kranz, Janusz Kowalik, Hideko Yamanaka, Boris Mizaikoff, and Mira JosowiczAnalytical Chemistry2008 80 (1), 237-245We present a new strategy for the label-free electrochemical detection of DNA hybridization for detecting hepatitis C virus based on electrostatic modulation of the ion-exchange kinetics of a polypyrrole film deposited at microelectrodes. Synthetic single-...
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- Received: August 03, 2009
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