The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting

Peihong Liang , Bhavin Adhyaru , Wright L. Pearson and Kathryn R. Williams
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200
J. Chem. Educ., 2006, 83 (2), p 294
DOI: 10.1021/ed083p294
Publication Date (Web): February 1, 2006
History, Education, and Documentation

Abstract

Students determine the binding constant of estradiol to bovine serum albumin using 3H-labeled estradiol. The estradiol is equilibrated with excess BSA, and subsequently a small fraction (ca. 1%) of the unbound hormone is extracted using solid-phase microextraction (SPME). The fibers are counted using a liquid scintillation counter. The experiment is suitable for laboratories in biophysical chemistry, instrumental analysis, and radiochemistry.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Physical Chemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Bioanalytical Chemistry
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  • Received: August 03, 2009

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