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Demonstration of Absorbance Using Digital Color Image Analysis and Colored Solutions

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Shane K. Kohl , James D. Landmark and Douglas F. Stickle

Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-3135

J. Chem. Educ., 2006, 83 (4), p 644

DOI: 10.1021/ed083p644

Publication Date (Web): April 1, 2006

Section:

History, Education, and Documentation

Abstract

This article describes a simple experiment in which the principle of absorbance may be demonstrated using digital color image analysis. Dilute yellow food coloring solutions in water were prepared in known relative concentrations and photographed in transparent cuvettes as a group placed against a diffuse fluorescent white light lightbox. Image analysis of the intensity of the complementary color (blue) for each solution produced data that conformed to the Beer–Lambert law.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Analytical Chemistry

Keywords (Feature):

Cost-Effective Teacher

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Aqueous Solution Chemistry

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