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Manual Solid–Phase Peptide Synthesis of Metallocene–Peptide Bioconjugates

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Srecko I. Kirin , Fozia Noor and Nils Metzler-Nolte

Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, D-69120 Heidelberg, Germany

Walter Mier

Department of Nuclear Medicine, University Hospital Heidelberg, D-69120 Heidelberg, Germany

J. Chem. Educ., 2007, 84 (1), p 108

DOI: 10.1021/ed084p108

Publication Date (Web): January 1, 2007

Section:

History, Education, and Documentation

Abstract

We describe a simple procedure for the manual solid-phase synthesis of peptides. No expensive or specialized equipment is needed. Moreover, a variety of markers can be attached to the N-terminus. We describe two organometallic markers and a fluroescent dye, in addition to an unlabeled derivative that serves as a comparison. The experiment described herein was successfully carried out by fourth-year undergraduate students of molecular biotechnology. By carrying out this experiment, the students gained first-hand experience for one of the most important modern synthesis techniques.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Subject):

Bioorganic Chemistry

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This article has been cited by 4 ACS Journal articles (4 most recent appear below).

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Labeling of the Neuropeptide Enkephalin with Functionalized Tris(pyrazolyl)borate Complexes: Solid-Phase Synthesis and Characterization of p-[Enk-OH]COC₆H₄TpPtMe₃ and p-[Enk-OH]COC₆H₄Tp^MeRe(CO)₃
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