Prev. Article Next Article Table of Contents

Article

Survey of Biochemical Separation Techniques

Your current credentials do not allow retrieval of the full text.

Purchase the full-text

PDF/HTML,
figures/images,
references and tables,
(where available)

Melanie R. Nilsson

Department of Chemistry, McDaniel College, Westminster, MD 21157

J. Chem. Educ., 2007, 84 (1), p 112

DOI: 10.1021/ed084p112

Publication Date (Web): January 1, 2007

Section:

History, Education, and Documentation

Abstract

The separation of a mixture of myoglobin and blue dextran is explored using five different biochemical separation techniques (syringe filtration, Centricon, dialysis, gel filtration, and solid-phase extraction). The mixture is green and separation is detected by the visual observation of the colored components (orange–red myoglobin and blue dextran). Students gain exposure and hands-on practice with a broad range of modern biochemical separation techniques and the entire exercise can easily be completed in one laboratory period.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Subject):

Chromatography

View: Hi-Res PDF | PDF w/ Links

Citing Articles

View all 1 citing articles

Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.

This article has been cited by 1 ACS Journal articles (1 most recent appear below).

What Is the True Color of Fresh Meat? A Biophysical Undergraduate Laboratory Experiment Investigating the Effects of Ligand Binding on Myoglobin Using Optical, EPR, and NMR Spectroscopy
Kimberly Linenberger, Stacey Lowery Bretz, Michael W. Crowder, Robert McCarrick, Gary A. Lorigan, and David L. Tierney
Journal of Chemical Education2011 88 (2), 223-225
- What Is the True Color of Fresh Meat? A Biophysical Undergraduate Laboratory Experiment Investigating the Effects of Ligand Binding on Myoglobin Using Optical, EPR, and NMR Spectroscopy
  Kimberly Linenberger, Stacey Lowery Bretz, Michael W. Crowder, Robert McCarrick, Gary A. Lorigan, and David L. Tierney
  Journal of Chemical Education2011 88 (2), 223-225
  With an increased focus on integrated upper-level laboratories, we present an experiment integrating concepts from inorganic, biological, and physical chemistry content areas. Students investigate the effects of ligand strength on the spectroscopic ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links