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Quantitative HPLC Analysis of Rosmarinic Acid in Extracts of Melissa officinalis and Spectrophotometric Measurement of Their Antioxidant Activities
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Abstract
The students prepare tea samples using different quantities of lemon balm leaves (Melissa officinalis) and measure the rosmarinic acid contents by an HPLC–DAD method. The antioxidant properties of the tea samples are evaluated by a spectrophotometric method using a radical-scavenging assay with DPPH· (2,2-diphenyl-1-picrylhydrazyl). Finally the students are asked to correlate the measured antioxidant properties of the different teas with their contents in rosmarinic acid.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Applications of ChemistryCiting Articles
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This article has been cited by 4 ACS Journal articles (4 most recent appear below).
Extraction and Purification of Glucoraphanin by Preparative High-Performance Liquid Chromatography (HPLC)
Iris Lee and Mary C. BoyceJournal of Chemical Education2011 88 (6), 832-834Extraction and Purification of Glucoraphanin by Preparative High-Performance Liquid Chromatography (HPLC)
Iris Lee and Mary C. BoyceJournal of Chemical Education2011 88 (6), 832-834A student activity that focuses on the isolation of glucoraphanin from broccoli using preparative high-performance liquid chromatography (HPLC) is presented here. Glucoraphanin is a glucosinolate, whose byproducts are known to possess anticancer ...
Demonstrating the Antioxidative Capacity of Substances with Lightsticks
Robert R. Wieczorek and Katrin SommerJournal of Chemical Education2011 88 (4), 468-469Demonstrating the Antioxidative Capacity of Substances with Lightsticks
Robert R. Wieczorek and Katrin SommerJournal of Chemical Education2011 88 (4), 468-469The antioxidative capacity of phytochemical compounds is often discussed in life science courses (to prevent or slow cancer) and food science courses (to prevent the oxidation of sensitive ingredients). Thus, we developed a laboratory experiment where the ...
Quantification of Tea Flavonoids by High Performance Liquid Chromatography
Jessica D. Freeman and Emily D. NiemeyerJournal of Chemical Education2008 85 (7), 951Quantification of Tea Flavonoids by High Performance Liquid Chromatography
Jessica D. Freeman and Emily D. NiemeyerJournal of Chemical Education2008 85 (7), 951We have developed a laboratory experiment that uses high performance liquid chromatography (HPLC) to quantify flavonoid levels in a variety of commercial teas. Specifically, this experiment analyzes a group of flavonoids known as catechins, plant-derived ...
Molecular Models of Rosmarinic Acid and DPPH
William F. ColemanJournal of Chemical Education2007 84 (9), 1496Molecular Models of Rosmarinic Acid and DPPH
William F. ColemanJournal of Chemical Education2007 84 (9), 1496This month's Featured Molecules are the free radical DPPH· (2,2-diphenyl-1-picrylhydrazyl) and the cis- and trans- isomers of the antioxidant rosmarinic acid.
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- Received: August 03, 2009
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