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Quantitative Determination of Photosynthetic Pigments in Green Beans Using Thin-Layer Chromatography and a Flatbed Scanner as Densitometer

Juan Valverde and Hervé This

Equipe INRA de Gastronomie Moléculaire, UMR 214 (INRA/AgroParisTech), Laboratoire de Chimie Analytique, 75005, Paris, France

Marc Vignolle

Marie Groupe UNIQ, 94526, Rungis, France

J. Chem. Educ., 2007, 84 (9), p 1505

DOI: 10.1021/ed084p1505

Publication Date (Web): September 1, 2007

Abstract

A simple method for the quantitative determination of photosynthetic pigments extracted from green beans using thin-layer chromatography is proposed. Various extraction methods are compared, and it is shown how a simple flatbed scanner and free software for image processing can give a quantitative determination of pigments.

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Graduate Education / Research

Keywords:

Analytical Chemistry

Keywords:

Computer-Based Learning

Keywords:

Bioanalytical Chemistry

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Planar Chromatography
Joseph Sherma
Analytical Chemistry2008 80 (12), 4253-4267
- Planar Chromatography
  Joseph Sherma
  Analytical Chemistry2008 80 (12), 4253-4267
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)
Juan Valverde and Hervé This
Journal of Agricultural and Food Chemistry2008 56 (2), 314-320
- ¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)
  Juan Valverde and Hervé This
  Journal of Agricultural and Food Chemistry2008 56 (2), 314-320
  Using 1H nuclear magnetic resonance spectroscopy (1D and 2D), the two types of photosynthetic pigments (chlorophylls, their derivatives, and carotenoids) of “green beans” (immature pods of Phaseolus vulgaris L.) were analyzed. Compared to other analytical ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links

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Received: August 03, 2009

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An Improved Method for the Extraction and Thin-Layer Chromatography of Chlorophyll a and b from SpinachJournal of Chemical Education
- An Improved Method for the Extraction and Thin-Layer Chromatography of Chlorophyll a and b from Spinach
  Hao T. Quach , Robert L. Steeper and G. William Griffin
  Journal of Chemical Education 2004 81 (3), p 385
  Abstract:
  A direct method of extracting plant pigments from spinach leaves into a dry organic solvent is presented. A powder is obtained by grinding a mixture of raw spinach, drying agent, and sand (1:1:2) that can be extracted directly into acetone in 10 minutes ...
  Abstract | PDF w/ Links | Hi-Res PDF
¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)Journal of Agricultural and Food Chemistry
- ¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)
  Juan Valverde and Hervé This
  Journal of Agricultural and Food Chemistry 2008 56 (2), pp 314–320
  Abstract: Using ¹H nuclear magnetic resonance spectroscopy (1D and 2D), the two types of photosynthetic pigments (chlorophylls, their derivatives, and carotenoids) of “green beans” (immature pods of Phaseolus vulgaris L.) were analyzed. Compared to other analytical ...
  Abstract | Full Text HTML | PDF w/ Links | Hi-Res PDF

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Article

Quantitative Determination of Photosynthetic Pigments in Green Beans Using Thin-Layer Chromatography and a Flatbed Scanner as Densitometer

Abstract

Keywords:

Keywords:

Keywords:

Keywords:

Citing Articles

Planar Chromatography

Planar Chromatography

¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)

¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)

Tools

SciFinder Links

History

Recommend & Share

Related Content

An Improved Method for the Extraction and Thin-Layer Chromatography of Chlorophyll a and b from Spinach

¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)

JOURNALS

BOOKS

TOOLS

MAGAZINES

COMMUNITIES

DIRECTORIES & DATABASES

Article

Quantitative Determination of Photosynthetic Pigments in Green Beans Using Thin-Layer Chromatography and a Flatbed Scanner as Densitometer

Abstract

Keywords:

Keywords:

Keywords:

Keywords:

Citing Articles

Planar Chromatography

Planar Chromatography

1H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)

1H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)

Tools

SciFinder Links

History

Recommend & Share

Related Content

An Improved Method for the Extraction and Thin-Layer Chromatography of Chlorophyll a and b from Spinach

1H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)

JOURNALS

BOOKS

TOOLS

MAGAZINES

COMMUNITIES

DIRECTORIES & DATABASES

¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)

¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)

¹H NMR Quantitative Determination of Photosynthetic Pigments from Green Beans (Phaseolus vulgaris L.)