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Illustrating the Concepts of Isotopes and Mass Spectrometry in Introductory Courses: A MALDI-TOF Mass Spectrometry Laboratory Experiment
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Abstract
Mass spectrometry is a widely used and versatile tool for scientists in many different fields. Soft ionization techniques such as matrix-assisted laser desorption/ionization (MALDI) allow for the analysis of biomolecules, polymers, and clusters. This article describes a MALDI mass spectrometry experiment designed for students in introductory courses that provides hands-on experience with research instrumentation and reinforces the course concepts of isotopes, molecular masses, and molecular formulas. The students prepare peptide samples for mass analysis and then collect and analyze mass spectral data. This experiment could easily be adapted to an instructor's available or preferred soft ionization technique.
Keywords (Audience):
First-Year Undergraduate / GeneralKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
IsotopesCiting Articles
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This article has been cited by 3 ACS Journal articles (3 most recent appear below).
Using the Mini-Session Course Format To Train Students in the Practical Aspects of Modern Mass Spectrometry
Dale A. Rosado, Jr., Tina S. Masterson, and Douglas S. MastersonJournal of Chemical Education2011 88 (2), 178-183Using the Mini-Session Course Format To Train Students in the Practical Aspects of Modern Mass Spectrometry
Dale A. Rosado, Jr., Tina S. Masterson, and Douglas S. MastersonJournal of Chemical Education2011 88 (2), 178-183Mass spectrometry (MS) has been gaining in popularity in recent years owing in large part to the development of soft-ionization techniques such as matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI). These soft-ionization ...
Utilization of Negative Ion ESI-MS and Tandem Mass Spectrometry To Detect and Confirm the NADH−Boric Acid Complex
Danny H. Kim, Curtis D. Eckhert, Kym F. FaullJournal of Chemical Education2011 88 (1), 106-110Utilization of Negative Ion ESI-MS and Tandem Mass Spectrometry To Detect and Confirm the NADH−Boric Acid Complex
Danny H. Kim, Curtis D. Eckhert, Kym F. FaullJournal of Chemical Education2011 88 (1), 106-110Mass spectrometry (MS) is a powerful analytical technique that is now widely used in the chemical, physical, engineering, and life sciences, with rapidly growing applications in many areas including clinical, forensic, pharmaceutical, and environmental ...
Identification of Fatty Acids, Phospholipids, and Their Oxidation Products Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry and Electrospray Ionization Mass Spectrometry
Christopher W. Harmon, Stephen A. Mang, John Greaves and Barbara J. Finlayson-PittsJournal of Chemical Education2010 87 (2), 186-189Identification of Fatty Acids, Phospholipids, and Their Oxidation Products Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry and Electrospray Ionization Mass Spectrometry
Christopher W. Harmon, Stephen A. Mang, John Greaves and Barbara J. Finlayson-PittsJournal of Chemical Education2010 87 (2), 186-189Electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) have found increasing application in the analysis of biological samples. Using these techniques to solve problems in analytical ...
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- Received: August 03, 2009
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