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Immobilized alpha-Galactosidase in the Biochemistry Laboratory

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V. H. Mulimani and K. Dhananjay

Department of Biochemistry, Gulbarga University, Gulbarga-585106, Karnataka, India

J. Chem. Educ., 2007, 84 (12), p 1974

DOI: 10.1021/ed084p1974

Publication Date (Web): December 1, 2007

Section:

History, Education, and Documentation

Abstract

This laboratory experiment was designed to demonstrate the application of immobilized galactosidase in food industry to hydrolyze raffinose family oligosaccharides in soymilk. This laboratory experiment was conducted for postgraduate students of biochemistry and developed for graduate and undergraduate students of biochemistry, biotechnology, chemical engineering, and food technology courses. The students were able to compare the hydrolysis of raffinose family oligosaccharides by free and immobilized-galactosidase. This experiment illustrates the basic concept of immobilization and its application.

Keywords (Audience):

Graduate Education / Research

Keywords (Domain):

Analytical Chemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Biotechnology

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

Biocatalysis with Sol−Gel Encapsulated Acid Phosphatase
Suhasini Kulkarni, Vu Tran, Maggie K.-M. Ho, Chieu Phan, Elizabeth Chin, Zeke Wemmer and Monika Sommerhalter
Journal of Chemical Education2010 87 (9), 958-960
- Biocatalysis with Sol−Gel Encapsulated Acid Phosphatase
  Suhasini Kulkarni, Vu Tran, Maggie K.-M. Ho, Chieu Phan, Elizabeth Chin, Zeke Wemmer and Monika Sommerhalter
  Journal of Chemical Education2010 87 (9), 958-960
  This experiment was performed in an upper-level undergraduate biochemistry laboratory course. Students learned how to immobilize an enzyme in a sol−gel matrix and how to perform and evaluate enzyme-activity measurements. The enzyme acid phosphatase (APase)...
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