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High-Pressure Liquid Chromatography: Quantitative Analysis of Chinese Herbal Medicine

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W. F. Chan and C. W. Lin

Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong

J. Chem. Educ., 2007, 84 (12), p 1982

DOI: 10.1021/ed084p1982

Publication Date (Web): December 1, 2007

Section:

History, Education, and Documentation

Abstract

An HPLC undergraduate experiment on the analysis of traditional Chinese medicine (TCM) has been developed. Two commonly used herbs (glycyrrhizae radix and cinnamomi ramulus) are studied. Glycyrrhizin, cinnamic acid, and cinnamaldehyde are chosen as markers for the herbs. The dried herbs in their natural state and a TCM preparation in powder form are analyzed. Gradient elution with diode-array detection is used for the separation and identification of the markers. Solid-phase extraction is employed for cleaning the sample solutions.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Analytical Chemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Drugs / Pharmaceuticals

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