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Isolation of Three Components from Spearmint Oil: An Exercise in Column and Thin-Layer Chromatography
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Abstract
Three major components of spearmint oil, (+)-limonene, L-(−)-carvone, and (1R,2R,4R)-dihydrocarveol, are separated by silica gel column chromatography. The separation is monitored by thin-layer chromatography (TLC), with IR analysis employed to verify the identity of the separated components. Spearmint oil was chosen because it is relatively nontoxic and the separation and analysis involves colorless components. Since most organic compounds are colorless liquids, this experiment gives students a more authentic laboratory experience in separations technology than experiments involving colored mixtures or compounds that are easily crystallized. In addition, students are required to use indirect visualization methods on a TLC plate to detect the major components of the original mixture and determine the success of the column chromatographic separation.
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Second-Year UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Subject):
Chirality / Optical ActivityCiting Articles
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This article has been cited by 5 ACS Journal articles (5 most recent appear below).
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- Received: August 03, 2009
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