A Colorimetric Analysis Experiment Not Requiring a Spectrophotometer: Quantitative Determination of Albumin in Powdered Egg White. A High School or General Science Experiment

Amanda K. Charlton , Richard S. Sevcik , Dorie A. Tucker and Linda D. Schultz
Department of Chemistry, Geosciences, and Environmental Science, Tarleton State University, Stephenville, TX 76402
J. Chem. Educ., 2007, 84 (5), p 826
DOI: 10.1021/ed084p826
Publication Date (Web): May 1, 2007

Abstract

Albumin in powdered egg white is reacted with bromophenol blue, and the resulting blue color is compared to that formed by an albumin standard using a visual comparator method.

Keywords (Audience):

High School / Introductory Chemistry

Keywords (Domain):

Analytical Chemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Bioanalytical Chemistry

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History

  • Received: August 03, 2009

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