Cart
Article
Quantitative Comparison of Three Standardization Methods Using a One-Way ANOVA for Multiple Mean Comparisons
Hi-Res PDFPurchase the full-text
- PDF/HTML,
figures/images,
references and tables,
(where available)
Abstract
The use of an external standard calibration curve, standard addition, and internal standard methods for quantitatively determining the concentration of analytes is important to the study of analytical chemistry, especially in the area of instrumental analysis. To give students exposure to these methods in a non-traditional, guided-inquiry exercise that can be performed in a single laboratory period, the experiment reported here asks students to determine the concentration of three long-chain hydrocarbons by all three standardization methods using an experimental procedure of their own design by gas chromatography (GC). To persuade students to compare and contrast the experimental requirements of the three standardization methods, they are given only ten GC vials and a single unknown sample containing the three paraffin analytes. Although the students do not know the concentrations of the three analytes in their unknown sample, they do know that each of the three standardization methods should generate similar values for each analyte because they are given a single unknown sample. To test whether they have been successful, the students perform a one-way ANOVA statistical analysis on their results to see if the null hypothesis should be accepted or rejected.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Alkanes / CycloalkanesTools
-
Add to Favorites
-
Download Citation
-
Email a Colleague -
Permalink
Order Reprints
Rights & Permissions
Citation Alerts
History
- Received: August 03, 2009
ACS
Network
Facebook
Tweet This
CiteULike
Newsvine
Digg This
Delicious
