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Rapid-Equilibrium Enzyme Kinetics

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Robert A. Alberty

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139

J. Chem. Educ., 2008, 85 (8), p 1136

DOI: 10.1021/ed085p1136

Publication Date (Web): August 1, 2008

Section:

History, Education, and Documentation

Abstract

Rapid-equilibrium rate equations for enzyme-catalyzed reactions are especially useful because if experimental data can be fit by these simpler rate equations, the Michaelis constants can be interpreted as equilibrium constants. However, for some reactions it is necessary to use the more complicated steady-state rate equations. Thermodynamics is used in rapid-equilibrium derivations to calculate the equilibrium concentrations of reactants up to the rate-determining reaction, and that means that the reactions in the mechanism have to be independent. This is quite different from steady-state treatments of mechanisms where reactions do not have to be independent. The innovation in this article is the use of half-reactions to construct complete rapid-equilibrium rate equations. Five half-reactions are described. Any forward half-reaction can be combined with any reverse half-reaction. Rapid-equilibrium rate equations are given for fifteen mechanisms of enzyme-catalyzed reactions, but more rate equations can be constructed from these five half-reactions.

Keywords (Audience):

Graduate Education / Research

Keywords (Domain):

Biochemistry

Keywords (Subject):

Bioenergetics

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

A Comprehensive Enzyme Kinetic Exercise for Biochemistry
Janice S. Barton
Journal of Chemical Education2011 88 (9), 1336-1339
- A Comprehensive Enzyme Kinetic Exercise for Biochemistry
  Janice S. Barton
  Journal of Chemical Education2011 88 (9), 1336-1339
  This article describes a comprehensive treatment of experimental enzyme kinetics strongly coupled to electronic data acquisition and use of spreadsheets to organize data and perform linear and nonlinear least-squares analyses, all in a manner that ...
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