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Analysis of Peppermint Leaf and Spearmint Leaf Extracts by Thin-Layer Chromatography

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Libbie S. W. Pelter , Andrea Amico , Natalie Gordon , Chylah Martin , Dessalyn Sandifer and Michael W. Pelter

Department of Chemistry and Physics, Purdue University Calumet, Hammond, IN 46323-2094

J. Chem. Educ., 2008, 85 (1), p 133

DOI: 10.1021/ed085p133

Publication Date (Web): January 1, 2008

Section:

History, Education, and Documentation

Abstract

In this inquiry-based activity, the usefulness of thin-layer chromatography (TLC) to visualize the difference between spearmint and peppermint is explored. The experiment may be used in any class where TLC is discussed from high school to college. We have used this activity with science majors in an organic chemistry laboratory, with non-science majors in a brewing science class, and in a general science class for elementary education majors. The experiment can be completed in a two-hour period. R-(-)-Carvone and (1R,2S,5R)-(-)-menthol are responsible for the cool and minty sensations associated with these plants. These observable properties and the structural formula of the compounds provide the basis for a good discussion on structure–activity relationships.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Natural Products

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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

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Molecular Models of Leaf Extracts
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Journal of Chemical Education2008 85 (1), 160
- Molecular Models of Leaf Extracts
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  The Featured Molecules this month come from the analysis of leaf extracts by thin-layer chromatography and include the principal constituents of spearmint and peppermint oil.
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