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Observation of DNA Molecules Using Fluorescence Microscopy and Atomic Force Microscopy
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Abstract
This article describes experiments for an undergraduate instrumental analysis laboratory that aim to observe individual double-stranded DNA (dsDNA) molecules using fluorescence microscopy and atomic force microscopy (AFM). dsDNA molecules are observed under several different conditions to discuss their chemical and physical properties. In particular, immobilization of dsDNA molecules on a planar substrate in their stretched form permits students to measure the size of the dsDNA molecules using these microscopic techniques. Comparison between the measured size and literature values provides a means for teaching the characteristics of the microscopic techniques. The experiments can be completed within four hours and are suitable to introduce these modern microscopic methods as well as the basics of nanotechnology to undergraduate students during limited lab hours.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Biophysical ChemistryCiting Articles
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This article has been cited by 3 ACS Journal articles (3 most recent appear below).
Atomic Force Microscopy Analysis of Nanocrystalline Patterns Fabricated Using Micromolding in Capillaries
Benjamin M. Lyman, Orrin J. Farmer, Ryan D. Ramsey, Samuel T. Lindsey, Stephanie Stout, Adam Robison, Holly J. Moore, and Wesley C. SandersJournal of Chemical Education2012 Article ASAPAtomic Force Microscopy Analysis of Nanocrystalline Patterns Fabricated Using Micromolding in Capillaries
Benjamin M. Lyman, Orrin J. Farmer, Ryan D. Ramsey, Samuel T. Lindsey, Stephanie Stout, Adam Robison, Holly J. Moore, and Wesley C. SandersJournal of Chemical Education2012 Article ASAPA cost-effective, hands-on laboratory exercise is described for demonstrating nanoscale fabrication at non-research-based educational institutions. The laboratory exercise also contains a component involving qualitative and quantitative surface ...
Incorporating Basic Optical Microscopy in the Instrumental Analysis Laboratory
Paul A. FlowersJournal of Chemical Education2011 88 (12), 1716-1719Incorporating Basic Optical Microscopy in the Instrumental Analysis Laboratory
Paul A. FlowersJournal of Chemical Education2011 88 (12), 1716-1719A simple and versatile approach to incorporating basic optical microscopy in the undergraduate instrumental analysis laboratory is described. Attaching a miniature CCD spectrometer to the video port of a standard compound microscope yields a visible ...
Atomic Force Microscopy: Opening the Teaching Laboratory to the Nanoworld
Ron Blonder, Ernesto Joselevich, Sidney R. CohenJournal of Chemical Education2010 87 (12), 1290-1293Atomic Force Microscopy: Opening the Teaching Laboratory to the Nanoworld
Ron Blonder, Ernesto Joselevich, Sidney R. CohenJournal of Chemical Education2010 87 (12), 1290-1293The atomic force microscope (AFM), first demonstrated in 1986, quickly became the workhorse of nanoscience, providing nanometer-scale imaging, local mechanical and electronic probing, and manipulation. The extensive use of AFM in research triggered ...
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- Received: August 03, 2009
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