Cart
Article
Size Exclusion Chromatography: An Experiment for High School and Community College Chemistry and Biotechnology Laboratory Programs
Hi-Res PDFPurchase the full-text
- PDF/HTML,
figures/images,
references and tables,
(where available)
Abstract
A simple multiday laboratory exercise suitable for use in a high school or community college chemistry course or a biotechnology advanced placement biology course is described. In this experiment students gain experience in the use of column chromatography as a tool for the separation and characterization of biomolecules, thus expanding their exposure to chromatographic procedures beyond those more commonly employed in the high school environment (e.g., paper or thin-layer chromatography). Students chromatographically separate a standard mixture of three highly colored compounds of varying molar mass (vitamin B12, bovine hemoglobin, and blue dextran) on a short Sephacryl S-300 size exclusion column, determining their relative elution volumes. Students later apply a sample of the enzyme wheat germ acid phosphatase to the same column, collect eluant fractions and determine the elution position of the enzyme by use of a rapid and simple enzyme assay carried out in a 96 well plate. The relative molar mass of the enzyme is estimated by comparison of its elution volume to the elution profile of the molecules in the standard mixture.
Keywords (Audience):
First-Year Undergraduate / GeneralKeywords (Domain):
BiochemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
BiotechnologyCiting Articles
Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.
This article has been cited by 1 ACS Journal articles (1 most recent appear below).
Polymerization Kinetics: Monitoring Monomer Conversion Using an Internal Standard and the Key Role of Sample t0
Olivier Colombani, Ophélie Langelier, and Ekkachaï Martwong , Patrice CastignollesJournal of Chemical Education2011 88 (1), 116-121Polymerization Kinetics: Monitoring Monomer Conversion Using an Internal Standard and the Key Role of Sample t0
Olivier Colombani, Ophélie Langelier, and Ekkachaï Martwong , Patrice CastignollesJournal of Chemical Education2011 88 (1), 116-121The use of an internal standard is a conventional and convenient way to monitor the conversion of one or several monomers during a controlled radical polymerization. However, the validity of this technique relies on an accurate determination of the ...
Full Text HTMLTools
-
Add to Favorites
-
Download Citation
-
Email a Colleague -
Permalink
Order Reprints
Rights & Permissions
Citation Alerts
SciFinder Links
Explore by:
History
- Received: August 03, 2009
ACS
Network
Facebook
Tweet This
CiteULike
Newsvine
Digg This
Delicious
