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A Simple Laboratory Experiment To Determine the Kinetics of Mutarotation of D-Glucose Using a Blood Glucose Meter

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Carlos E. Perles and Pedro L. O. Volpe

Institute of Chemistry, State University of Campinas, CEP 13084-971 Campinas, São Paulo, Brazil

J. Chem. Educ., 2008, 85 (5), p 686

DOI: 10.1021/ed085p686

Publication Date (Web): May 1, 2008

Abstract

A simple commercial blood glucose meter is used to follow the kinetics of mutarotation of D-glucose in aqueous solution. The results may be compared with those obtained using an automatic polarimeter, if this is available This experiment is proposed for use by students in a general chemistry, biology, organic chemistry, and physical chemistry laboratory at the college level. It can also be used as an alternative technique to the classical methods that use relatively expensive equipment, which are not always accessible to students. The technique of indirect measurement of the kinetics of mutarotation of D-glucose through an enzymatic redox reaction also provides an example of the chemical and kinetic approaches to the mutarotation phenomenon and the principle of enzymatic stereospecificity. The test strips of the blood glucose meter contain glucose dehydrogenase, an enzyme specific for the anomer of D-glucose in solution.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Analytical Chemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Aqueous Solution Chemistry

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Kinetic Parameters for the Noncatalyzed and Enzyme-Catalyzed Mutarotation of Glucose Using a Blood Glucometer
John R. Hardee, Bryan Delgado, and Wray Jones
Journal of Chemical Education2011 Article ASAP
- Kinetic Parameters for the Noncatalyzed and Enzyme-Catalyzed Mutarotation of Glucose Using a Blood Glucometer
  John R. Hardee, Bryan Delgado, and Wray Jones
  Journal of Chemical Education2011 Article ASAP
  The kinetic parameters for the conversion of α-d-glucose to β-d-glucose were measured using a blood glucometer. The reaction order, rate constant, and Arrhenius activation energy are reported for the noncatalyzed reaction and turnover number and Michaelis ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
Crystallization of β-d-Glucose and Analysis with a Simple Glucose Biosensor
José I. Reyes-de-Corcuera , Michael A. Teruel and Daniel M. Jenkins
Journal of Chemical Education2009 86 (8), 959
- Crystallization of β-d-Glucose and Analysis with a Simple Glucose Biosensor
  José I. Reyes-de-Corcuera , Michael A. Teruel and Daniel M. Jenkins
  Journal of Chemical Education2009 86 (8), 959
  A simple method to crystallize β-D-glucose using reagents and equipment common to any chemistry laboratory is described. The crystals are analyzed with a glucose electrode prepared by entrapping glucose oxidase in a poly-ortho-phenylenediamine film on a ...
  Abstract | Hi-Res PDF | PDF w/ Links