Using the Enzymatic Growth of Nanoparticles To Create a Biosensor. An Undergraduate Quantitative Analysis Experiment

James Bai , Kaleyhia Flowers , Salil Benegal , Milagros Calizo , Vrudhdhi Patel and Sandra Whaley Bishnoi
Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago, IL 60616
J. Chem. Educ., 2009, 86 (6), p 712
DOI: 10.1021/ed086p712
Publication Date (Web): June 1, 2009

Abstract

This second-year analytical chemistry laboratory exposes students to biosensors and the concepts of sensitivity, linear range, and dynamic range. The use of gold nanoparticles provides a method of peroxide (Part I) and glucose (Part II) sensing that can be monitored colorimetrically using either a single wavelength or scanning UV–vis spectrophotometer. Each part of the experiment can be conducted in triplicate within a three-hour laboratory period or both combined can be conducted within the three-hour period.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Analytical Chemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Bioanalytical Chemistry
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History

  • Received: August 03, 2009

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