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A Theoretical Analysis of the Influence of Electroosmosis on the Effective Ionic Mobility in Capillary Zone Electrophoresis

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Hens Hijnen

Engineering and Applied Science, Rotterdam University, 3001 HA Rotterdam, The Netherlands

J. Chem. Educ., 2009, 86 (7), p 869

DOI: 10.1021/ed086p869

Publication Date (Web): July 1, 2009

Abstract

A theoretical description of the influence of electroosmosis on the effective mobility of simple ions in capillary zone electrophoresis is presented. The mathematical equations derived from the space-charge model contain the pK_a value and the density of the weak acid surface groups as parameters characterizing the capillary. It is shown how the zeta potential of the capillary and the effective mobility of typical anions are affected by the pH and ionic strength of the background electrolyte. Furthermore, the model is also used to analyze experimental results on the pH dependency of the electroosmotic mobility in a fused silica capillary, yielding estimates for the pK_a values of two types of silanol groups and the total surface density of these groups . With these estimated values for the properties of the silanol groups, the model predictions of the effective mobility are compared with literature data.

Keywords (Audience):

Graduate Education / Research

Keywords (Domain):

Analytical Chemistry

Keywords (Feature):

Research: Science and Education

Keywords (Pedagogy):

Textbooks / Reference Books

Keywords (Subject):

Electrophoresis

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
Lisa A. Holland
Journal of Chemical Education2011 88 (3), 254-256
- Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
  Lisa A. Holland
  Journal of Chemical Education2011 88 (3), 254-256
  Capillary electrophoresis is a versatile technique well suited to teach concepts that are fundamental to a chemistry degree program. The applicability of capillary electrophoresis to metabolites, DNA, proteins, pharmaceutical compounds, environmental ...
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