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Crystallization of β-d-Glucose and Analysis with a Simple Glucose Biosensor

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José I. Reyes-de-Corcuera

Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850

Michael A. Teruel and Daniel M. Jenkins

Department of Molecular Biosciences and Bioengineering, University of Hawai''i at MÄnoa, Honolulu, HI 96822

J. Chem. Educ., 2009, 86 (8), p 959

DOI: 10.1021/ed086p959

Publication Date (Web): August 1, 2009

Abstract

A simple method to crystallize β-D-glucose using reagents and equipment common to any chemistry laboratory is described. The crystals are analyzed with a glucose electrode prepared by entrapping glucose oxidase in a poly-ortho-phenylenediamine film on a platinized working electrode. Mutarotation between α and β anomers of the sugar results in continuous changes in current until equilibrium is reached. Since the electrode is selective to the β anomer, dissolution of β-D-glucose crystals results in an observable overshoot of the equilibrium current value, and dissolution of α-D-glucose crystals initially results in little or no current. The lab is targeted to first-year college students, some of whom have little or no science training. Analysis of student data indicates that the D-glucose crystallized in the lab was almost purely in the β conformation.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Laboratory Instruction

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Bioanalytical Chemistry

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