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Using Proteins in a Bioinorganic Laboratory Experiment: Iron Loading and Removal from Transferrin

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David H. Hamilton

Department of Chemistry, Rockhurst University, Kansas City, MO 64110

Erin E. Battin , Ashley Lawhon and Julia L. Brumaghim

Department of Chemistry, Clemson University, Clemson, SC 29634

J. Chem. Educ., 2009, 86 (8), p 969

DOI: 10.1021/ed086p969

Publication Date (Web): August 1, 2009

Abstract

With the increasing availability of metalloproteins it is now possible to incorporate them into bioinorganic laboratory experiments. Thus, we have developed a laboratory experiment where students use UV–vis spectroscopy to determine the rate of iron removal from transferrin by a well-known, commercially-available iron chelating drug, Deferiprone. Students gain experience in both chemical and biological laboratory techniques, while assessing the efficacy of a drug that is physiologically relevant to humans.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Aqueous Solution Chemistry

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

UV–Visible Spectrometers: Versatile Instruments across the Chemistry Curriculum
Demetra A. C. Czegan and Diana K. Hoover
Journal of Chemical Education2012 Article ASAP
- UV–Visible Spectrometers: Versatile Instruments across the Chemistry Curriculum
  Demetra A. C. Czegan and Diana K. Hoover
  Journal of Chemical Education2012 Article ASAP
  This article describes a wide range of commercially available UV–vis spectrometers, with particular emphasis on educational models, and how this technique can be included in all aspects of the chemistry curriculum. Portable instrumentation and new ...
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An Undergraduate Laboratory Experiment in Bioinorganic Chemistry: Ligation States of Myoglobin
James A. Bailey
Journal of Chemical Education2011 Article ASAP
- An Undergraduate Laboratory Experiment in Bioinorganic Chemistry: Ligation States of Myoglobin
  James A. Bailey
  Journal of Chemical Education2011 Article ASAP
  Although there are numerous inorganic model systems that are readily presented as undergraduate laboratory experiments in bioinorganic chemistry, there are few examples that explore the inorganic chemistry of actual biological molecules. We present a ...
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