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Article

Effect of a Fullerene Water Suspension on Bacterial Phospholipids and Membrane Phase Behavior

Jiasong Fang,^†* Delina Y. Lyon,^‡ Mark R. Wiesner,^§ Jinping Dong, and Pedro J.J. Alvarez^‡

Department of Geological and Atmospheric Sciences, Iowa State University, Ames, Iowa 50011, Department of Civil and Environmental Engineering, Rice University, Houston, Texas 77005, Department of Civil and Environmental Engineering, Duke University, Durham, North Carolina 27708, and Characterization Facility, Institute of Technology, University of Minnesota, Minneapolis, Minnesota 55455

Environ. Sci. Technol., 2007, 41 (7), pp 2636–2642

DOI: 10.1021/es062181w

Publication Date (Web): February 22, 2007

†

Iowa State University.

Corresponding author phone: 515-294-6583; fax: 515-294-6049; e-mail: jsfang@iastate.edu.

‡

Rice University.

Duke University.

University of Minnesota.

Abstract

Several fullerene-based nanomaterials generate reactive oxygen species that can damage cells. In this study, we investigated the effect of buckminsterfullerene (C₆₀) introduced as colloidal aggregates in water (nC₆₀) on bacterial membrane lipid composition and phase behavior. Pseudomonas putida (Gram-negative) and Bacillus subtilis (Gram-positive) responded to nC₆₀ by altering membrane lipid composition, phase transition temperature, and membrane fluidity. P. putida decreased its levels of unsaturated fatty acids and increased the proportions of cyclopropane fatty acids in the presence of nC₆₀, possibly to protect the bacterial membrane from oxidative stress. Fourier transform infrared spectroscopy measurement of intact bacterial cells showed slightly increased phase transition temperatures (T_m) and increased membrane fluidity for cells grown in the presence of high, growth-inhibiting concentrations (0.5 mg L^-1) of nC₆₀. B. subtilis responded to a low dose of nC₆₀ (0.01 mg L^-1) by significantly increasing the levels of iso- and anteiso-branched fatty acids (from 5.8 to 31.5% and 12.9 to 32.3% of total fatty acids, respectively) and to a high, growth-inhibiting concentration of nC₆₀ (0.75 mg L^-1) by increasing synthesis of monounsaturated fatty acids. In contrast to P. putida, B. subtilis response was a decrease in T_m and an increase in membrane fluidity. These findings represent the first demonstrated physiological adaptation response of bacteria to a manufactured nanomaterial, and they show that response in lipid composition and membrane phase behavior depends on both the nC₆₀ concentration and the cell wall morphology.