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Communication

Intein-Mediated Biotinylation of Proteins and Its Application in a Protein Microarray

Supporting Info

Marie-Laure Lesaicherre,^† Rina Y. P. Lue,^‡ Grace Y. J. Chen,^†^‡ Qing Zhu,^† and Shao Q. Yao*^†^‡

Department of Chemistry and Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore

J. Am. Chem. Soc., 2002, 124 (30), pp 8768–8769

DOI: 10.1021/ja0265963

Publication Date (Web): July 2, 2002

†

Department of Chemistry.

‡

Department of Biological Sciences.

Address correspondence to this author. E-mail: chmyaosq@nus.edu.sg. Tel: 65-68741683. Fax: 65-67791691.

Abstract

We report here the first example using an intein-mediated expression system to generate biotinylated proteins suitable for immobilization onto avidin-functionalized glass slides. With this novel array, proteins are site-specifically immobilized on the glass surface and are able to retain their native activity. The advantage of the avidin/biotin linkage over his-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety of chemical conditions, which makes this new protein array compatible with most biological assays.

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