An Addressable Antibody Nanoarray Produced on a Nanostructured Surface

Andreas Bruckbauer,† Dejian Zhou,† Dae-Joon Kang,‡ Yuri E. Korchev,§ Chris Abell,† and David Klenerman*†
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K., Nanoscience Center, IRC in Nanotechnology, University of Cambridge, 11 J J Thomson Avenue, Cambridge CB3 0FF, U.K., and Division of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, U.K.
J. Am. Chem. Soc., 2004, 126 (21), pp 6508–6509
DOI: 10.1021/ja0317426
Publication Date (Web): May 8, 2004
Copyright © 2004 American Chemical Society
†

 Department of Chemistry, University of Cambridge.

,
‡

 Nanoscience Center, University of Cambridge.

,
§

 Imperial College London.

,
*

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

, dk10012@cam.ac.uk

Abstract

Abstract Image

The ability to address specific nanoscale features is required to produce diverse biological nanoarrays or perform local assembly using biological building blocks and is an important unsolved problem in nanotechnology. In this work, we describe the use of a novel nanofabricated gold surface, with regions of distinct topography and chemical functionalities, to solve this problem. First, nanoarrays of IgG antibodies were produced by selective immobilization in nanoholes on the surface. The smallest feature size was determined by the hole size (fwhm 90 nm) and not surface diffusion. Using holes of 300 nm diameter, we selectively addressed specific features in the array by nanopipet delivery of a functional antibody, anti-IgG. To our knowledge, this is the first example of addressing specific biologically functional features on a surface at the nanoscale.

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History

  • Published In Issue June 02, 2004
  • Received December 16, 2003

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