Novel Fluorogenic Substrates for Imaging β-Lactamase Gene Expression

Wenzhong Gao,† Bengang Xing,† Roger Y. Tsien,‡ and Jianghong Rao*†
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095-1770, and Howard Hughes Medical Institute, Departments of Pharmacology and Chemistry & Biochemistry, University of California San Diego, La Jolla, California 92093-0647
J. Am. Chem. Soc., 2003, 125 (37), pp 11146–11147
DOI: 10.1021/ja036126o
Publication Date (Web): August 19, 2003
Copyright © 2003 American Chemical Society
†

 University of California, Los Angeles.

,
‡

 University of California, San Diego.

,
*

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

, jhrao@mednet.ucla.edu

Abstract

Abstract Image

A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after β-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of β-lactamase in cell lysates can be readily detected, and β-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of β-lactamase as a biosensor.

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History

  • Published In Issue September 17, 2003
  • Received May 13, 2003

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