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Communication

Near-Infrared Fluorescence Microscopy of Single-Walled Carbon Nanotubes in Phagocytic Cells

Supporting Info

Paul Cherukuri,^†^§ Sergei M. Bachilo,^‡ Silvio H. Litovsky,^† and R. Bruce Weisman*^‡

Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, and Department of Chemistry, Center for Biological and Environmental Nanotechnology, and Center for Nanoscale Science and Technology, Rice University, 6100 Main Street, Houston, Texas 77005

J. Am. Chem. Soc., 2004, 126 (48), pp 15638–15639

DOI: 10.1021/ja0466311

Publication Date (Web): November 11, 2004

†

University of Texas Health Science Center.

Current address: Department of Chemistry, Rice University.

‡

Rice University.

Current address: Department of Pathology, School of Medicine, University of Alabama at Birmingham.

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

, weisman@rice.edu

Abstract

The uptake of pristine single-walled carbon nanotubes into macrophage-like cells has been studied using the nanotubes' intrinsic near-infrared fluorescence. Macrophage samples that have been incubated in growth media containing suspended single-walled nanotubes show characteristic nanotube fluorescence spectra. The fluorescence intensities increase smoothly with incubation time and external nanotube concentration. Near-infrared fluorescence microscopy at wavelengths above 1100 nm provides high contrast images indicating localization of nanotubes in numerous intracellular vesicles. Nanotube uptake appears to occur through phagocytosis. Population growth of macrophage cultures is unaffected by exposure to single-walled nanotube concentrations of ca. 4 μg/mL for up to 96 h.

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Published In Issue December 08, 2004
Received June 8, 2004

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Communication

Near-Infrared Fluorescence Microscopy of Single-Walled Carbon Nanotubes in Phagocytic Cells

Abstract

Tools

SciFinder Links

History

Recommend & Share

Related Content

Nanotube Molecular Transporters: Internalization of Carbon Nanotube−Protein Conjugates into Mammalian Cells

Carbon Nanotubes as Intracellular Protein Transporters: Generality and Biological Functionality

Chemistry of Single-Walled Carbon Nanotubes

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