Prev. Article Next Article Table of Contents

Article

In Vivo Magnetic Resonance Detection of Cancer by Using Multifunctional Magnetic Nanocrystals

Supporting Info

Yong-Min Huh,^†^‡ Young-wook Jun,^†^§ Ho-Taek Song,^†^‡ Sungjun Kim,^†^‡ Jin-sil Choi,^†^§ Jae-Hyun Lee,^†^§ Sarah Yoon,^¶ Kyung-Sup Kim,^¶ Jeon-Soo Shin,^†^¶ Jin-Suck Suh,*^†^‡ and Jinwoo Cheon*^†^§;

Contribution from the Yonsei Nano-Medical Core Research Center, Department of Radiology, Department of Chemistry, Department of Biochemistry and Molecular Biology, and Department of Microbiology, Yonsei University, Seoul 120-752, Korea

J. Am. Chem. Soc., 2005, 127 (35), pp 12387–12391

DOI: 10.1021/ja052337c

Publication Date (Web): August 16, 2005

†

Yonsei Nano-Medical Core Research.

‡

Department of Radiology.

Department of Chemistry.

Department of Biochemistry and Molecular Biology.

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

, jss@yumc.yonsei.ac.kr, ; , jcheon@yonsei.ac.kr

Abstract

The unique properties of magnetic nanocrystals provide them with high potential as key probes and vectors in the next generation of biomedical applications. Although superparamagnetic iron oxide nanocrystals have been extensively studied as excellent magnetic resonance imaging (MRI) probes for various cell trafficking, gene expression, and cancer diagnosis, further development of in vivo MRI applications has been very limited. Here, we describe in vivo diagnosis of cancer, utilizing a well-defined magnetic nanocrystal probe system with multiple capabilities, such as small size, strong magnetism, high biocompatibility, and the possession of active functionality for desired receptors. Our magnetic nanocrystals are conjugated to a cancer-targeting antibody, Herceptin, and subsequent utilization of these conjugates as MRI probes has been successfully demonstrated for the monitoring of in vivo selective targeting events of human cancer cells implanted in live mice. Further conjugation of these nanocrystal probes with fluorescent dye-labeled antibodies enables both in vitro and ex vivo optical detection of cancer as well as in vivo MRI, which are potentially applicable for an advanced multimodal detection system. Our study finds that high performance in vivo MR diagnosis of cancer is achievable by utilizing improved and multifunctional material properties of iron oxide nanocrystal probes.

View: Full Text HTML | Hi-Res PDF