Prev. Article Next Article Table of Contents

Communication

A Cellular FRET-Based Sensor for β-O-GlcNAc, A Dynamic Carbohydrate Modification Involved in Signaling

Supporting Info

Luz D. Carrillo, Lakshmipriya Krishnamoorthy, and Lara K. Mahal*

Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station, A5300 Austin, Texas 78712-0265

J. Am. Chem. Soc., 2006, 128 (46), pp 14768–14769

DOI: 10.1021/ja065835+

Publication Date (Web): October 28, 2006

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

, lmahal@cm.utexas.edu

Abstract

β-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.

View: Full Text HTML | Hi-Res PDF