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Dumbbell-Shaped Nanocircular RNAs for RNA Interference

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Naoko Abe, Hiroshi Abe,* and Yoshihiro Ito*;

Nano Medical Engineering Laboratory, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako-Shi, Saitama 351-0198, Japan

J. Am. Chem. Soc., 2007, 129 (49), pp 15108–15109

DOI: 10.1021/ja0754453

Publication Date (Web): November 15, 2007

Section:

General Biochemistry

Abstract

We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops. RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.

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Citing Articles

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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

Synthesis, Structure, and Biological Activity of Dumbbell-Shaped Nanocircular RNAs for RNA Interference
Naoko Abe, Hiroshi Abe, Chisato Nagai, Mitsuru Harada, Hiroto Hatakeyama, Hideyoshi Harashima, Takahito Ohshiro, Mizuki Nishihara, Kazuhiro Furukawa, Mizuo Maeda, Satoshi Tsuneda, and Yoshihiro Ito
Bioconjugate Chemistry2011 22 (10), 2082-2092
- Synthesis, Structure, and Biological Activity of Dumbbell-Shaped Nanocircular RNAs for RNA Interference
  Naoko Abe, Hiroshi Abe, Chisato Nagai, Mitsuru Harada, Hiroto Hatakeyama, Hideyoshi Harashima, Takahito Ohshiro, Mizuki Nishihara, Kazuhiro Furukawa, Mizuo Maeda, Satoshi Tsuneda, and Yoshihiro Ito
  Bioconjugate Chemistry2011 22 (10), 2082-2092
  RNA interference (RNAi) is one of the most promising new approaches for disease therapy. The design of a dumbbell-shaped nanocircular RNA allows it to act as a short interfering RNA (siRNA) precursor. To optimize the design, we studied the relationship ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
Self-Assembling RNA Nanorings Based on RNAI/II Inverse Kissing Complexes
Wade W. Grabow, Paul Zakrevsky, Kirill A. Afonin, Arkadiusz Chworos, Bruce A. Shapiro, and Luc Jaeger
Nano Letters2011 11 (2), 878-887
- Self-Assembling RNA Nanorings Based on RNAI/II Inverse Kissing Complexes
  Wade W. Grabow, Paul Zakrevsky, Kirill A. Afonin, Arkadiusz Chworos, Bruce A. Shapiro, and Luc Jaeger
  Nano Letters2011 11 (2), 878-887
  RNA is an attractive biopolymer for nanodesign of self-assembling particles for nanobiotechnology and synthetic biology. Here, we experimentally characterize by biochemical and biophysical methods the formation of thermostable and ribonuclease resistant ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
Unprecedented C-Selective Interstrand Cross-Linking through in Situ Oxidation of Furan-Modified Oligodeoxynucleotides
Marieke Op de Beeck and Annemieke Madder
Journal of the American Chemical Society2011 133 (4), 796-807
- Unprecedented C-Selective Interstrand Cross-Linking through in Situ Oxidation of Furan-Modified Oligodeoxynucleotides
  Marieke Op de Beeck and Annemieke Madder
  Journal of the American Chemical Society2011 133 (4), 796-807
  Chemical reagents that form interstrand cross-links have been used for a long time in cancer therapy. They covalently link two strands of DNA, thereby blocking transcription. Cross-link repair enzymes, however, can restore the transcription processes, ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links