Detection of Sequence-Specific Protein-DNA Interactions via Surface Enhanced Resonance Raman Scattering

Andrew J. Bonham, Gary Braun, Ioana Pavel, Martin Moskovits, and Norbert O. Reich*
Department of Chemistry and Biochemistry, The University of California Santa Barbara, Santa Barbara, California 93106-9510
J. Am. Chem. Soc., 2007, 129 (47), pp 14572–14573
DOI: 10.1021/ja0767837
Publication Date (Web): November 7, 2007
Copyright © 2007 American Chemical Society
*

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

, reich@chem.ucsb.edu

Abstract

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We developed a new strategy to detect protein-DNA binding through surface enhanced resonance Raman scattering (SERRS). Silver-plated DNA and gold nanoparticle assemblies were used to identify sequence-specific and concentration-dependent binding of a DNA cytosine-C5-methyltransferase and the eukaryotic transcriptional regulator, TATA binding protein. Proteins were identified through specific Raman-active labels, and affinities observed correlate well with those determined by other methods. Raman-active labeling and interchangeable DNA sequences create a platform for versatile investigation of multiprotein complexes.

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History

  • Published In Issue November 28, 2007
  • Received September 7, 2007

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