Surface-Enhanced Raman Spectroscopy of DNA

Aoune Barhoumi†, Dongmao Zhang‡, Felicia Tam§ and Naomi J. Halas†‡
Departments of Chemistry, Electrical and Computer Engineering, and Physics and Astronomy and the Laboratory for Nanophotonics, Rice University, Houston, Texas 77005
J. Am. Chem. Soc., 2008, 130 (16), pp 5523–5529
DOI: 10.1021/ja800023j
Publication Date (Web): March 29, 2008
Copyright © 2008 American Chemical Society
†

Department of Chemistry.

,

Laboratory for Nanophotonics.

,
‡

Department of Electrical and Computer Engineering.

,
§

Department of Physics and Astronomy.

Abstract

Abstract Image

We report a method for obtaining highly reproducible surface-enhanced Raman spectroscopy (SERS) of single and double-stranded thiolated DNA oligomers. Following a protocol that relaxes the DNA into an extended conformation, SERS spectra of DNA oligonucleotides are found to be extremely similar, strongly dominated by the Stokes modes of adenine. A spectral correlation function analysis useful for assessing reproducibility and for quantifying the highly complex changes corresponding to modifications in molecular conformation of the adsorbate molecules is introduced. This approach is used to monitor the interaction of DNA with cisplatin, a chemotherapy agent in widespread use.

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History

  • Published In Issue April 23, 2008
  • Article ASAPMarch 29, 2008
  • Received: January 02, 2008

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