Detection of Intact Influenza Viruses using Biotinylated Biantennary S-Sialosides

Ramesh R. Kale†, Harshini Mukundan‡, Dominique N. Price§, J. Foster Harris§, Daniel M. Lewallen†, Basil I. Swanson‡, Jurgen G. Schmidt§ and Suri S. Iyer†
Chemical and Biosensors group, 805 Crosley, Department of Chemistry, University of Cincinnati, Cincinnati, Ohio 45221, Physical Chemistry and Applied Spectroscopy (C-PCS), Los Alamos National Laboratory, P.O. Box 1663, MS J567, Los Alamos, New Mexico 87545, and Bioscience Division (B-7 and B-9), Los Alamos National Laboratory, PO Box 1663, MS E 529, Los Alamos, New Mexico 87545
J. Am. Chem. Soc., 2008, 130 (26), pp 8169–8171
DOI: 10.1021/ja800842v
Publication Date (Web): June 5, 2008
Copyright © 2008 American Chemical Society
†

University of Cincinnati.

,
‡

Physical Chemistry and Applied Spectroscopy (C-PCS), Los Alamos National Laboratory.

,
§

Bioscience Division (B-7 and B-9), Los Alamos National Laboratory.

Abstract

Abstract Image

We report the modular synthesis of robust, biotinylated biantennary sialylglycoconjugates and their ability to differentiate between two type A influenza strains. This is the first demonstration of glycoconjugate-based discriminatory capture and detection of two strains of intact influenza virus, in the presence of the innate enzymatic activity of viral neuraminidases. We also demonstrate a “carboassay” using glycoconjugates as capture and reporter elements, which therefore, does not require antibodies. The capture of intact influenza viruses is of potential benefit for clinical diagnostics.

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History

  • Published In Issue July 02, 2008
  • Article ASAPJune 05, 2008
  • Received: February 07, 2008

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