Magnetic Nanoparticle−Peptide Conjugates for in Vitro and in Vivo Targeting and Extraction of Cancer Cells

• Abstract
• Full Text HTML
• Hi-Res PDF[799 KB]
• PDF w/ Links[216 KB]

• Supporting Info
• Figures
• Citing Articles

Your current credentials do not allow retrieval of the full text.

Purchase the full-text

• PDF/HTML,
  figures/images,
  references and tables,
  (where available)

Abstract

Magnetic cobalt spinel ferrite nanoparticles coated with biocompatible polygalacturonic acid were functionalized with ligands specific for targeting expressed EphA2 receptors on ovarian cancer cells. By using such magnetic nanoparticle−peptide conjugates, targeting and extraction of malignant cells were achieved with a magnetic field. Targeting ovarian cancer cells with receptor specific peptide-modified magnetic nanoparticles resulted in cell capture from a flow stream in vitro and from the peritoneal cavity of mice in vivo. Successful removal of metastatic cancer cells from the abdominal cavity and circulation using magnetic nanoparticle conjugates indicate the feasibility of a dialysis-like treatment and may improve long-term survival rates of ovarian cancer patients. This approach can be applied for fighting other cancers, such as leukemia, once the receptors on malignant cells are identified and the efficacy of targeting ligands is established.

Citing Articles

View all 8 citing articles

Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.

This article has been cited by 8 ACS Journal articles (5 most recent appear below).

• 

  A Direct Technique for Magnetic Functionalization of Living Human Cells

  Maria R. Dzamukova, Alsu I. Zamaleeva, Dilara G. Ishmuchametova, Yuri N. Osin, Andrey P. Kiyasov, Danis K. Nurgaliev, Olga N. Ilinskaya, and Rawil F. Fakhrullin
  Langmuir2011 27 (23), 14386-14393

  • A Direct Technique for Magnetic Functionalization of Living Human Cells

    Maria R. Dzamukova, Alsu I. Zamaleeva, Dilara G. Ishmuchametova, Yuri N. Osin, Andrey P. Kiyasov, Danis K. Nurgaliev, Olga N. Ilinskaya, and Rawil F. Fakhrullin
    Langmuir2011 27 (23), 14386-14393

    Functionalized living cells are regarded as effective tools in directed cell delivery and tissue engineering. Here we report the facile functionalization of viable isolated HeLa cells with superparamagnetic cationic nanoparticles via a single-step ...

    Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
• 

  Small-Angle Neutron Scattering Investigation of Gold Nanoparticle Clustering and Ligand Structure Under Antisolvent Conditions

  Gregory Von White, II, Fiaz S. Mohammed, and Christopher L. Kitchens
  The Journal of Physical Chemistry C2011 Article ASAP

  • Small-Angle Neutron Scattering Investigation of Gold Nanoparticle Clustering and Ligand Structure Under Antisolvent Conditions

    Gregory Von White, II, Fiaz S. Mohammed, and Christopher L. Kitchens
    The Journal of Physical Chemistry C2011 Article ASAP

    Solution-based nanoparticle synthesis offers many benefits, primarily control over nanoparticle size, shape, and surface chemistry, as appropriate for different applications. Often, excess stabilizing ligand or surfactant is required during synthesis and ...

    Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
• 

  Nanoshape Control Tendency of Phospholipids and Proteins: Protein–Nanoparticle Composites, Seeding, Self-Aggregation, and Their Applications in Bionanotechnology and Nanotoxicology

  Mandeep Singh Bakshi
  The Journal of Physical Chemistry C2011 Article ASAP

  • Nanoshape Control Tendency of Phospholipids and Proteins: Protein–Nanoparticle Composites, Seeding, Self-Aggregation, and Their Applications in Bionanotechnology and Nanotoxicology

    Mandeep Singh Bakshi
    The Journal of Physical Chemistry C2011 Article ASAP

    This feature article studies different areas related to phospholipid/protein conjugated nanoparticles ranging from their basic synthetic strategies of shape-directed morphologies to their applications in biodegradable materials and nanotoxicology. Present ...

    Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
• 

  Size Effects on Adsorption of Hematite Nanoparticles on E. coli cells

  Wen Zhang, Bruce Rittmann, and Yongsheng Chen
  Environmental Science & Technology2011 45 (6), 2172-2178

  • Size Effects on Adsorption of Hematite Nanoparticles on E. coli cells

    Wen Zhang, Bruce Rittmann, and Yongsheng Chen
    Environmental Science & Technology2011 45 (6), 2172-2178

    Adsorption of engineered nanoparticles (NPs) onto bacterial cells is critical for quantifying nanobio interactions as well as toxicokinetic properties of NPs. The purpose of this work was to study adsorption of hematite (α-Fe2O3) NPs onto Escherichia coli ...

    Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
• 

  Structure−Activity Relationship Analysis of Peptides Targeting the EphA2 Receptor

  Sayantan Mitra, Srinivas Duggineni, Mitchell Koolpe, Xuejun Zhu, Ziwei Huang and Elena B. Pasquale
  Biochemistry2010 49 (31), 6687-6695

  • Structure−Activity Relationship Analysis of Peptides Targeting the EphA2 Receptor

    Sayantan Mitra, Srinivas Duggineni, Mitchell Koolpe, Xuejun Zhu, Ziwei Huang and Elena B. Pasquale
    Biochemistry2010 49 (31), 6687-6695

    The EphA2 receptor tyrosine kinase has emerged as a promising new therapeutic target in cancer because of its high level of expression in tumors. EphA2-specific antibodies have been used to deliver drugs and toxins to tumor cells, leading to inhibition of ...

    Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links

SciFinder Links

• Get Reference Detail
• Get Substances
• Get Cited
• Get Citing